7EGT

The crystal structure of the C-terminal domain of T. thermophilus UvrD complexed with the N-terminal domain of UvrB

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-05-22
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97776
Spacegroup name	P 21 21 21
Unit cell lengths	92.332, 114.606, 125.448
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.166 - 2.581
R-factor	0.2166
Rwork	0.215
R-free	0.24930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1d2m
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.640
High resolution limit [Å]	2.580	7.050	2.600
Rmerge	0.118	0.039	0.992
Rmeas	0.129	0.042	1.106
Rpim	0.051	0.017	0.476
Number of reflections	41725	2293	1883
<I/σ(I)>	5.7
Completeness [%]	98.6	99.4	90.9
Redundancy	6.1	6	4.8
CC(1/2)		0.999	0.704

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M BIS-TRIS, pH 6.1, 15 % w/v Polyethylene glycol 1500