7EGS

The crystal structure of lobe domain of E. coli RNA polymerase complexed with the C-terminal domain of UvrD

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U1
Synchrotron site	SSRF
Beamline	BL17U1
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97853
Spacegroup name	P 1 21 1
Unit cell lengths	60.085, 42.241, 87.849
Unit cell angles	90.00, 105.23, 90.00

Refinement procedure

Resolution	37.807 - 1.700
R-factor	0.2048
Rwork	0.203
R-free	0.23360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3lti
RMSD bond length	0.008
RMSD bond angle	1.058
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	40.000	40.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.065	0.047	0.405
Rmeas	0.077	0.056	0.485
Rpim	0.041	0.030	0.263
Number of reflections	46583	2433	2294
<I/σ(I)>	16.3
Completeness [%]	98.9	98.6	99.4
Redundancy	3.3	3.4	3.2
CC(1/2)		0.991	0.853

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M HEPES pH 7.0, 10% w/v polyethylene glycol 4000, 10 % (v/v) isopropanol