7EGO
X-ray structure of the human heart fatty acid-binding protein complexed with the fluorescent probe HA527
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.521, 69.657, 33.844 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.930 - 1.210 |
| R-factor | 0.13034 |
| Rwork | 0.129 |
| R-free | 0.15159 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wvm |
| RMSD bond length | 0.022 |
| RMSD bond angle | 2.293 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0238) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.930 | 1.280 |
| High resolution limit [Å] | 1.210 | 1.210 |
| Rmerge | 0.130 | 0.500 |
| Number of reflections | 40169 | 4825 |
| <I/σ(I)> | 17.77 | 5.1 |
| Completeness [%] | 94.5 | |
| Redundancy | 10.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 0.1M Tris-HCl (pH8.5), 50% PEG 400 |
