7EG5
FMN-bound form of YviC from Lactococcus lactis subsp. lactis Il1403
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-X |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2020-10-29 |
| Detector | RIGAKU RAXIS VII |
| Wavelength(s) | 1.5418 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 41.509, 94.801, 142.075 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.930 - 1.960 |
| R-factor | 0.2 |
| Rwork | 0.199 |
| R-free | 0.22800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1flm |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.609 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.930 | 2.070 |
| High resolution limit [Å] | 1.960 | 1.960 |
| Rmerge | 0.070 | 0.378 |
| Number of reflections | 20447 | |
| <I/σ(I)> | 16.1 | |
| Completeness [%] | 99.3 | 98.7 |
| Redundancy | 8.2 | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M HEPES (pH 7.62), 1.7 M Ammonium sulfate |
