7EFW
Crystal structure of hexameric state of C-phycocyanin from Thermoleptolyngbya sp. O-77
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL45XU |
| Synchrotron site | SPring-8 |
| Beamline | BL45XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-10-17 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 2 21 21 |
| Unit cell lengths | 60.089, 187.439, 210.065 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.180 - 1.650 |
| R-factor | 0.1706 |
| Rwork | 0.171 |
| R-free | 0.19010 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1i7y |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.980 |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.18.2_3874: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.200 | 1.740 |
| High resolution limit [Å] | 1.650 | 1.650 |
| Rmerge | 0.194 | |
| Rmeas | 0.221 | 1.062 |
| Rpim | 0.083 | |
| Number of reflections | 264074 | 25606 |
| <I/σ(I)> | 8.6 | |
| Completeness [%] | 92.4 | 65.1 |
| Redundancy | 5.9 | 2.9 |
| CC(1/2) | 0.993 | 0.495 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5 | 293.15 | 8% Tacsimate pH 5.0, 20 % Polyethylene glycol (PEG) 3350 |
