7EFW

Crystal structure of hexameric state of C-phycocyanin from Thermoleptolyngbya sp. O-77

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL45XU
Synchrotron site	SPring-8
Beamline	BL45XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-10-17
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1
Spacegroup name	P 2 21 21
Unit cell lengths	60.089, 187.439, 210.065
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.180 - 1.650
R-factor	0.1706
Rwork	0.171
R-free	0.19010
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1i7y
RMSD bond length	0.005
RMSD bond angle	0.980
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX ((1.18.2_3874: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.200	1.740
High resolution limit [Å]	1.650	1.650
Rmerge	0.194
Rmeas	0.221	1.062
Rpim	0.083
Number of reflections	264074	25606
<I/σ(I)>	8.6
Completeness [%]	92.4	65.1
Redundancy	5.9	2.9
CC(1/2)	0.993	0.495

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5	293.15	8% Tacsimate pH 5.0, 20 % Polyethylene glycol (PEG) 3350