7EFJ
Crystal Structure Analysis of human PIN1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-23 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 68.177, 68.177, 79.724 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 33.040 - 1.992 |
| Rwork | 0.190 |
| R-free | 0.22100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ntp |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.12) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.450 | 2.040 |
| High resolution limit [Å] | 1.990 | 1.990 |
| Rmerge | 0.056 | 0.231 |
| Number of reflections | 15079 | 1053 |
| <I/σ(I)> | 32.6 | |
| Completeness [%] | 99.7 | |
| Redundancy | 17.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 277.15 | 2M Ammonium Sulfate, 1% PEG400, 100mM HEPES, pH 7.5 |
