7EFI

RNA kink-turn motif composed of RNA, DNA and 2'-O-methyl RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 63 2 2
Unit cell lengths	70.565, 70.565, 48.165
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	37.830 - 2.900
R-factor	0.1968
Rwork	0.192
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4cs1
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	37.830	37.830	2.980
High resolution limit [Å]	2.900	12.290	2.900
Rmerge	0.057	0.030	0.366
Rmeas	0.065	0.035	0.415
Total number of observations	7974
Number of reflections	1761	28	142
<I/σ(I)>	16.91	28.04	3.98
Completeness [%]	98.5	82.4	99.3
Redundancy	4.528	3.179	4.451
CC(1/2)	0.999	1.000	0.956

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium cacodylate, MPD, spermine tetrahydrochloride, potassium chloride