7EF7

Crystal Structure of Xanthosine monophosphate phosphatase complex with XMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2018-05-09
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97933
Spacegroup name	P 1 21 1
Unit cell lengths	37.670, 56.779, 56.958
Unit cell angles	90.00, 92.92, 90.00

Refinement procedure

Resolution	25.402 - 1.500
R-factor	0.2138
Rwork	0.213
R-free	0.23980
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7ef6
RMSD bond length	0.009
RMSD bond angle	1.169
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.530
High resolution limit [Å]	1.500	1.500
Number of reflections	37816
<I/σ(I)>	15.2
Completeness [%]	98.3
Redundancy	3.5
CC(1/2)	0.989	0.989

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.1 M HEPES (pH 7.0) 18%(w/v) PEG12000