7EF0
Crystal structure of maize SHH2 SAWADEE domain in complex with an H3K9M peptide
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2015-05-24 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 46.405, 50.032, 133.677 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.119 - 1.500 |
| R-factor | 0.1808 |
| Rwork | 0.179 |
| R-free | 0.20820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7eez |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.070 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.550 |
| High resolution limit [Å] | 1.500 | 3.230 | 1.500 |
| Rmerge | 0.102 | 0.064 | 0.558 |
| Rmeas | 0.110 | 0.069 | 0.596 |
| Rpim | 0.039 | 0.025 | 0.209 |
| Total number of observations | 405599 | ||
| Number of reflections | 51003 | 5415 | 5040 |
| <I/σ(I)> | 8.4 | ||
| Completeness [%] | 100.0 | 99.8 | 100 |
| Redundancy | 8 | 7.4 | 8 |
| CC(1/2) | 0.996 | 0.885 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Bicine, pH 8.5, 25% PEG 300 |
