7EEY
The structure of the N-terminal doamin of the Schizosaccharomyces pombe Tad2 adenosine deaminase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-11-30 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 |
| Unit cell lengths | 62.527, 65.602, 65.642 |
| Unit cell angles | 63.97, 69.96, 73.56 |
Refinement procedure
| Resolution | 34.700 - 2.600 |
| R-factor | 0.1704 |
| Rwork | 0.168 |
| R-free | 0.21810 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1udw |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.775 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.680 |
| High resolution limit [Å] | 2.590 | 2.590 |
| Rmerge | 0.070 | 0.150 |
| Number of reflections | 26761 | 2603 |
| <I/σ(I)> | 33.2 | 15.7 |
| Completeness [%] | 98.7 | 97.4 |
| Redundancy | 7.1 | 7.1 |
| CC(1/2) | 0.994 | 0.986 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 298 | 20% PEG2000, 0.1 M NaOAc, 0.2 M (NH4)2SO4 |
