7EEW

Crystal structure of the intact MTase from Vibrio vulnificus YJ016 in complex with the DNA-mimicking Ocr protein and the S-adenosyl-L-homocysteine (SAH)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 11C
Synchrotron site	PAL/PLS
Beamline	11C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-03
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	P 65 2 2
Unit cell lengths	133.280, 133.280, 236.808
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	14.956 - 2.896
R-factor	0.2726
Rwork	0.272
R-free	0.29320
Structure solution method	SAD
RMSD bond length	0.002
RMSD bond angle	0.505
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.12_2829)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.950
High resolution limit [Å]	2.896	2.896
Rmerge	0.188	0.758
Rmeas	0.195	0.837
Rpim	0.050	0.343
Number of reflections	27924	1327
<I/σ(I)>	13.6
Completeness [%]	98.6
Redundancy	13.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	293	20% (w/v) polyethylene glycol 6000, 0.1 M sodium chloride, 0.1 M HEPES pH 8.5