7EEN

Bulged-G motif composed of RNA, DNA and 2'-O-methyl RNA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-04
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 43 21 2
Unit cell lengths	68.867, 68.867, 116.016
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.280 - 1.700
R-factor	0.1915
Rwork	0.189
R-free	0.23730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1q9a
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	30.280	30.280	1.740
High resolution limit [Å]	1.700	7.600	1.700
Rmerge	0.086	0.055	0.365
Rmeas	0.093	0.060	0.394
Total number of observations	201904
Number of reflections	30956	373	2246
<I/σ(I)>	11.97	21.19	4.7
Completeness [%]	98.2	87.1	98.3
Redundancy	6.522	5.753	6.805
CC(1/2)	0.996	0.998	0.940

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	sodium cacodylate, MPD, spermine tetrahydrochloride, calcium chloride