7EE2
Structural insights into the substrate-binding mechanism of a glycoside hydrolase family 12 beta-1,3-1,4-glucanase from Chaetomium sp.CQ31
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-01-10 |
| Detector | DECTRIS PILATUS3 R CdTe 300K |
| Wavelength(s) | 0.9792 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.801, 64.902, 84.310 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.227 - 1.370 |
| R-factor | 0.189029628041 |
| Rwork | 0.188 |
| R-free | 0.20639 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1olr |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.797 |
| Data reduction software | PHENIX (dev_2474) |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (dev_2474) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.230 | 1.419 |
| High resolution limit [Å] | 1.370 | 1.370 |
| Rmerge | 0.064 | 0.064 |
| Number of reflections | 55541 | 55541 |
| <I/σ(I)> | 55.587 | |
| Completeness [%] | 99.0 | |
| Redundancy | 1.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.0075M Nike(II) chloride hexahydrate, 0.075M Tris pH 8.5, 0.75M Lithium sulfate monohydrate, 25% glycerol |
