7EDR
The crystal structure of the FERM and C-terminal domain complex of Drosophila Merlin
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL19U1 |
Synchrotron site | SSRF |
Beamline | BL19U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-12-15 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9778 |
Spacegroup name | P 2 21 21 |
Unit cell lengths | 86.464, 100.366, 163.593 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.976 - 2.527 |
R-factor | 0.1866 |
Rwork | 0.184 |
R-free | 0.23500 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4zrj |
RMSD bond length | 0.009 |
RMSD bond angle | 0.933 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.10_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.570 |
High resolution limit [Å] | 2.527 | 2.530 |
Rpim | 0.047 | |
Number of reflections | 48350 | 2354 |
<I/σ(I)> | 14.2 | |
Completeness [%] | 100.0 | |
Redundancy | 6.4 | |
CC(1/2) | 0.892 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Sodium cacodylate/Hydrochloric acid, pH 6.5, 1.0 M sodium citrate tribasic |