7EDO

First insight into marsupial MHC I peptide presentation: immune features of lower mammals paralleled with bats

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-10-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.97918
Spacegroup name	I 2 3
Unit cell lengths	193.633, 193.633, 193.633
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.410 - 2.700
R-factor	0.1833
Rwork	0.180
R-free	0.23870
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vz5
RMSD bond length	0.009
RMSD bond angle	1.036
Data scaling software	HKL-2000 (1.13_2998)
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.800
High resolution limit [Å]	2.700	2.700
Rmerge	0.129	0.877
Number of reflections	33061	33061
<I/σ(I)>	2.69
Completeness [%]	99.9
Redundancy	38.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291.15	0.1 M Na citrate trisbasic dihydrate (pH 5.0) ,30%v/v Jeffamine ED-2001