7EDN

Crystal structure of the eukaryotic decoding site in complex with Ag(I)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-12-23
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	C 1 2 1
Unit cell lengths	51.176, 28.102, 37.711
Unit cell angles	90.00, 102.35, 90.00

Refinement procedure

Resolution	36.840 - 2.600
R-factor	0.1939
Rwork	0.188
R-free	0.25180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3td0
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	36.840	36.840	2.680
High resolution limit [Å]	2.600	10.720	2.600
Rmerge	0.107	0.084	0.328
Rmeas	0.128	0.102	0.389
Total number of observations	10577
Number of reflections	3135	45	268
<I/σ(I)>	6.88	10.53	2.49
Completeness [%]	99.2	95.7	98.2
Redundancy	3.374	3.222	3.392
CC(1/2)	0.983	0.979	0.941

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MOPS, MPD, spermine, calcium nitrate, silver nitrate, G418