7ED6

Crystal structure of Thermus thermophilus FakA ATP-binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B1
Synchrotron site	SPring-8
Beamline	BL26B1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-17
Detector	DECTRIS EIGER X 4M
Wavelength(s)	0.9788
Spacegroup name	P 43 21 2
Unit cell lengths	90.190, 90.190, 111.620
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.095 - 1.929
R-factor	0.20945075519
Rwork	0.208
R-free	0.23641
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.753
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.10.1_2155)
Refinement software	PHENIX (1.10.1_2155)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.095	2.000
High resolution limit [Å]	1.928	1.928
Number of reflections	35300	3455
<I/σ(I)>	13	1.04
Completeness [%]	99.9	99.4
Redundancy	14.2	14.2
CC(1/2)	0.999	0.671

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	2.2M sodium chloride, 0.1M tris-HCl