7ECL

RNA duplex containing C-Ag-A and U-Ag-A base pair

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-10-26
Detector	DECTRIS EIGER X 4M
Wavelength(s)	1.1
Spacegroup name	P 32 2 1
Unit cell lengths	43.252, 43.252, 378.967
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.110 - 3.010
R-factor	0.2255
Rwork	0.218
R-free	0.29250
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.17.1)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	42.110	42.110	3.080
High resolution limit [Å]	3.010	13.450	3.010
Rmerge	0.081	0.028	0.330
Rmeas	0.092	0.034	0.370
Total number of observations	41779
Number of reflections	9049	136	669
<I/σ(I)>	10.6	19.84	3.2
Completeness [%]	99.5	96.5	98.2
Redundancy	4.617	3.015	4.91
CC(1/2)	0.999	1.000	0.993

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	MOPS, MPD, spermine, lithium nitrate