7ECD

Crystal structure of Tam41 from Firmicutes bacterium, complex with CTP-Mg

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-17
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.92
Spacegroup name	I 41
Unit cell lengths	104.159, 104.159, 113.959
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.120 - 2.600
R-factor	0.2026
Rwork	0.201
R-free	0.23790
Structure solution method	SAD
RMSD bond length	0.008
RMSD bond angle	1.065
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX ((1.17_3644: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.760
High resolution limit [Å]	2.600	2.600
Number of reflections	36822	5901
<I/σ(I)>	23.8
Completeness [%]	99.7
Redundancy	13.5
CC(1/2)	0.999	0.722

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.1M HEPES ph 7.0, 13-15% (w/v) PEG 6000, 10% (v/v) Glycerol