7EBD
Bacterial STING in complex with c-di-GMP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE TPS 05A |
| Synchrotron site | NSRRC |
| Beamline | TPS 05A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-08-20 |
| Detector | RAYONIX MX300-HS |
| Wavelength(s) | 0.97836 |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 94.534, 86.995, 35.223 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 21.780 - 2.250 |
| R-factor | 0.1911 |
| Rwork | 0.188 |
| R-free | 0.24870 |
| Structure solution method | MAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.549 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.330 |
| High resolution limit [Å] | 2.250 | 2.250 |
| Rmerge | 0.106 | 0.615 |
| Rpim | 0.036 | 0.215 |
| Number of reflections | 14459 | 1410 |
| <I/σ(I)> | 22.6 | 3.8 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 9.3 | 9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.1M sodium cacodylate trihydrate pH 6.5, 0.2M sodium acetate trihydrate, 18% w/v polyethylene glycol 8000 |
