7EAW

Trehalase of Arabidopsis thaliana acid mutant -D380A trehalose complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL41XU
Synchrotron site	SPring-8
Beamline	BL41XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-06
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0
Spacegroup name	P 21 21 21
Unit cell lengths	52.671, 104.507, 237.842
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.160 - 1.800
R-factor	0.2285
Rwork	0.228
R-free	0.25810
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7e9u
RMSD bond length	0.007
RMSD bond angle	1.008
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.160	1.864
High resolution limit [Å]	1.800	1.800
Number of reflections	122983	19210
<I/σ(I)>	14.45
Completeness [%]	98.1
Redundancy	9.7
CC(1/2)	0.995	0.924

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	10 mM trehalose, 0.1 M sodium fluoride and 20% PEG3400