7EAV

The X-ray crystallographic structure of glycogen debranching enzyme from Sulfolobus solfataricus STB09

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-25
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	P 3 2 1
Unit cell lengths	203.543, 203.543, 89.466
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.113 - 2.803
R-factor	0.2674
Rwork	0.264
R-free	0.32520
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2vr5
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.14_3260)
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.890
High resolution limit [Å]	2.800	2.800
Rpim	0.162
Number of reflections	52466	4530
<I/σ(I)>	6.4
Completeness [%]	99.9
Redundancy	11.8
CC(1/2)		0.595

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	PEG3000,lithium sulfate, imidazole