7EAR
A positively charged mutant Cry3Aa endotoxin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-11-25 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 114.840, 132.890, 103.130 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 66.540 - 2.200 |
| R-factor | 0.182 |
| Rwork | 0.180 |
| R-free | 0.21650 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1dlc |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.652 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 66.540 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.099 | 0.552 |
| Number of reflections | 38330 | 5821 |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 99.9 | 99.9 |
| Redundancy | 7.2 | 7.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291.15 | 100mM HEPES/Sodium hydroxide pH7.5, 20% v/v 1,4-butanediol, 200mM Sodium chloride |
