7EAA
crystal structure of NDP52 SKICH domain in complex with RB1CC1 coiled-coil domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U1 |
Synchrotron site | SSRF |
Beamline | BL17U1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-05-26 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97919 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 69.439, 69.439, 246.095 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.050 - 2.600 |
R-factor | 0.2017 |
Rwork | 0.199 |
R-free | 0.25340 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5z7a |
RMSD bond length | 0.009 |
RMSD bond angle | 0.910 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.600 | 2.600 |
Rmerge | 0.158 | 2.815 |
Number of reflections | 19751 | 1942 |
<I/σ(I)> | 23.65 | |
Completeness [%] | 99.7 | |
Redundancy | 17.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 289 | 0.1 M MgCl2, 0.1 M MES pH 6.0, 8% PEG6000 |