7E9W

The Crystal Structure of D-psicose-3-epimerase from Biortus.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CLSI BEAMLINE 08ID-1
Synchrotron site	CLSI
Beamline	08ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-05
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.5215
Spacegroup name	P 1 21 1
Unit cell lengths	79.134, 116.083, 91.817
Unit cell angles	90.00, 105.08, 90.00

Refinement procedure

Resolution	48.607 - 2.100
Rwork	0.174
R-free	0.20580
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3vni
RMSD bond length	0.004
RMSD bond angle	1.220
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.607	2.140
High resolution limit [Å]	2.100	2.100
Number of reflections	91449	4468
<I/σ(I)>	10.2
Completeness [%]	98.0
Redundancy	3.4
CC(1/2)	0.998	0.829

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.02M MgCl2, 0.1M HEPES pH7.5, 22% Polyacrylic acid sodium salt 5,100