7E9J

Crystal Structure of POMGNT2 in complex with UDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-17A
Synchrotron site	Photon Factory
Beamline	BL-17A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2016-11-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.0718
Spacegroup name	P 21 21 21
Unit cell lengths	96.129, 106.233, 132.359
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.300 - 2.400
R-factor	0.2065
Rwork	0.205
R-free	0.23790
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.626
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.300	2.486
High resolution limit [Å]	2.400	2.400
Rmerge	0.247	1.858
Rpim	0.070	0.518
Number of reflections	56436	5292
<I/σ(I)>	10.5
Completeness [%]	100.0
Redundancy	13.4
CC(1/2)	0.997	0.442

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Tris-HCl (pH 8.0 or 8.5), 12-16 % PEG 8000, 1 % tacsimate (pH 7.0)