7E9D
H102A mutant of IRG1 from bacillus
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 5C (4A) |
Synchrotron site | PAL/PLS |
Beamline | 5C (4A) |
Temperature [K] | 110 |
Detector technology | PIXEL |
Collection date | 2020-08-12 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.97950 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.911, 110.779, 168.564 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.350 - 1.890 |
R-factor | 0.1735 |
Rwork | 0.172 |
R-free | 0.20210 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7bra |
RMSD bond length | 0.006 |
RMSD bond angle | 0.774 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.890 | 1.890 |
Number of reflections | 85234 | 85146 |
<I/σ(I)> | 18.7 | |
Completeness [%] | 95.6 | |
Redundancy | 9.8 | |
CC(1/2) | 0.999 | 0.798 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 297 | 1.4M ammonium sulfate, 0.1M CAPS pH 10.2, 0.2M lithium sulfate |