7E8L

The structure of Spodoptera litura chemosensory protein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007 HF
Temperature [K]	100
Detector technology	CCD
Collection date	2020-11-25
Detector	OXFORD ONYX CCD
Wavelength(s)	1.54
Spacegroup name	P 21 21 21
Unit cell lengths	38.400, 50.055, 60.238
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	23.710 - 2.300
R-factor	0.2108
Rwork	0.208
R-free	0.26070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n8u
RMSD bond length	0.008
RMSD bond angle	1.309
Data reduction software	CrysalisPro
Data scaling software	CrysalisPro
Phasing software	PHASER
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.700	2.420
High resolution limit [Å]	2.300	2.300
Rmerge	0.062	0.196
Number of reflections	5508	776
<I/σ(I)>	17.9	6.4
Completeness [%]	99.7	99
Redundancy	4.1	3.5
CC(1/2)	0.998	0.958

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	5.5	277	3M (NH4)2SO4, 0.1M NaOAc pH5.5