7E73
Crystal structure of human ERK2 mutant (Y36H)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
Synchrotron site | PAL/PLS |
Beamline | 7A (6B, 6C1) |
Temperature [K] | 93 |
Detector technology | CCD |
Collection date | 2020-08-03 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 0.987 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 48.857, 70.606, 60.905 |
Unit cell angles | 90.00, 109.65, 90.00 |
Refinement procedure
Resolution | 30.060 - 2.280 |
R-factor | 0.1843 |
Rwork | 0.178 |
R-free | 0.23920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1ERK |
RMSD bond length | 0.008 |
RMSD bond angle | 0.933 |
Data reduction software | HKL-2000 (715) |
Data scaling software | HKL-2000 (715) |
Phasing software | PHENIX (1.16-3549) |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.060 | 2.360 |
High resolution limit [Å] | 2.279 | 2.279 |
Rmerge | 0.073 | 0.174 |
Rmeas | 0.088 | 0.219 |
Rpim | 0.048 | 0.132 |
Number of reflections | 17017 | 1505 |
<I/σ(I)> | 10.95 | 3.69 |
Completeness [%] | 94.9 | 85.03 |
Redundancy | 2.8 | 2.1 |
CC(1/2) | 0.994 | 0.914 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 291 | 100mM HEPES pH 7.0, 1.4M ammonium sulfate, 13% glycerol |