7E6V

The crystal structure of foot-and-mouth disease virus(FMDV) 2C protein 97-318aa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-19
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.979081
Spacegroup name	P 1
Unit cell lengths	36.720, 40.737, 72.937
Unit cell angles	77.22, 84.38, 89.96

Refinement procedure

Resolution	38.480 - 1.832
R-factor	0.2031
Rwork	0.201
R-free	0.24900
Structure solution method	SAD
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX (1.13_2998)
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	39.719	1.940
High resolution limit [Å]	1.832	1.840
Rmerge	0.069	0.618
Number of reflections	67455	10786
<I/σ(I)>	7.75	1.23
Completeness [%]	93.5	93
Redundancy	1.69	1.65
CC(1/2)	0.996	0.597

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.6	296	0.09M Ammonium acetate, 0.045M Sodium citrate tribasic dihydrate pH 5.6, 13.5%w/v PEG 4000, 4% PEG P400, 5mM TECP