7E6G

Crystal structure of diguanylate cyclase SiaD in complex with its activator SiaC from Pseudomonas aeruginosa

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-02
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9785
Spacegroup name	C 2 2 21
Unit cell lengths	82.525, 236.728, 148.627
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.295 - 2.650
R-factor	0.2201
Rwork	0.218
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i5a
Data reduction software	DENZO
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.740
High resolution limit [Å]	2.650	2.650
Number of reflections	40897	4204
<I/σ(I)>	13.1
Completeness [%]	99.5
Redundancy	13.3
CC(1/2)	0.982	0.588

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		277	0.01 M Spermidine trihydrochloride and 15% w/v PEG 3350