7E5U

Crystal structure of Phm7

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-01-12
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.999994
Spacegroup name	C 1 2 1
Unit cell lengths	91.206, 150.481, 99.333
Unit cell angles	90.00, 97.35, 90.00

Refinement procedure

Resolution	43.867 - 1.620
R-factor	0.191
Rwork	0.189
R-free	0.22150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	SeMet derivative
RMSD bond length	0.014
RMSD bond angle	1.261
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER (2.7.17)
Refinement software	PHENIX ((1.11.1_2575: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	43.867	1.678
High resolution limit [Å]	1.620	1.620
Number of reflections	167533	16704
<I/σ(I)>	6.9
Completeness [%]	99.8
Redundancy	3.5
CC(1/2)	0.988	0.674

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	293	1.54-1.62M Ammonium sulfate, 0.10M Tris HCl, 14-19% (v/v) Glycerol