7E4V

Crystal structure of mosquito Staufen dsRNA binding domain 4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-1
Synchrotron site	NSLS-II
Beamline	17-ID-1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-10
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.978
Spacegroup name	P 32 2 1
Unit cell lengths	42.528, 42.528, 155.611
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.830 - 1.370
R-factor	0.1932
Rwork	0.192
R-free	0.21270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cff
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.390
High resolution limit [Å]	1.370	1.370
Rpim	0.033	0.398
Number of reflections	34672	1364
<I/σ(I)>	37.3
Completeness [%]	97.9
Redundancy	10.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		278	PEG3350 25%