7E4K
WNV envelope protein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-04-08 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 74.795, 34.930, 93.672 |
| Unit cell angles | 90.00, 112.99, 90.00 |
Refinement procedure
| Resolution | 45.790 - 2.690 |
| R-factor | 0.2617 |
| Rwork | 0.260 |
| R-free | 0.28800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hg0 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.865 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.690 | 2.700 |
| Number of reflections | 372171 | 12609 |
| <I/σ(I)> | 17.6 | |
| Completeness [%] | 99.4 | 100 |
| Redundancy | 7.3 | |
| CC(1/2) | 0.989 | 0.951 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 0.2 M NaI, 0.1 M Bis Tris propane, 20% w/v PEG 3350, 10 mg/ml |
