7E4G
Crystal structure of schizorhodopsin 4
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-04-10 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 1.0000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 106.000, 61.140, 98.750 |
| Unit cell angles | 90.00, 99.35, 90.00 |
Refinement procedure
| Resolution | 49.560 - 2.100 |
| R-factor | 0.2037 |
| Rwork | 0.202 |
| R-free | 0.23680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1m0k |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19_4092) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.560 | 2.175 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmerge | 0.384 | 3.282 |
| Rmeas | 0.392 | 3.369 |
| Rpim | 0.074 | 0.722 |
| Number of reflections | 36552 | 3615 |
| <I/σ(I)> | 6.81 | 1.18 |
| Completeness [%] | 99.6 | 98.42 |
| Redundancy | 21 | 19.2 |
| CC(1/2) | 0.988 | 0.390 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 293 | 20% PEG500DME, 100 mM Na-acetate, pH 4.75, 250 mM MgSO4. and 10 mM ZnSO4 |
