7E3Y

Crystal structure of serine acetyltransferase from Salmonella typhimurium complexed with cysteine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.90000
Spacegroup name	P 32 2 1
Unit cell lengths	121.946, 121.946, 127.674
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	19.740 - 1.900
R-factor	0.17211
Rwork	0.171
R-free	0.18879
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1t3d
RMSD bond length	0.010
RMSD bond angle	1.423
Data reduction software	XDS
Data scaling software	XDS
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0049)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	2.010
High resolution limit [Å]	1.900	1.900
Rmerge	0.070	0.471
Rmeas	0.072	0.488
Number of reflections	86865	13719
<I/σ(I)>	24.74	5.5
Completeness [%]	99.8	98.8
Redundancy	15.1	14.4
CC(1/2)	0.999	0.979

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	293	100 mM HEPES, 100 mM Sodium chloride, 6% MPD