7E3R
Crystal structure of TrmL from Vibrio vulnificus
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-05-11 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.97957 |
| Spacegroup name | P 41 21 2 |
| Unit cell lengths | 52.290, 52.290, 119.689 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.410 - 1.790 |
| R-factor | 0.2023 |
| Rwork | 0.200 |
| R-free | 0.24250 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4kdz |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.581 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 50.000 | 50.000 | 1.810 |
| High resolution limit [Å] | 1.780 | 4.830 | 1.780 |
| Rmerge | 0.080 | 0.040 | 0.833 |
| Rmeas | 0.084 | 0.042 | 0.875 |
| Rpim | 0.025 | 0.012 | 0.258 |
| Total number of observations | 174716 | ||
| Number of reflections | 16324 | 950 | 749 |
| <I/σ(I)> | 7 | ||
| Completeness [%] | 99.4 | 99.6 | 93.3 |
| Redundancy | 10.7 | 11.9 | 7.5 |
| CC(1/2) | 0.999 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 298 | 1M Tris-hydrochloride pH 8.5, 8% w/v PEG 8000 |
