7E2P

The Crystal Structure of Mycoplasma bovis enolase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL19U1
Synchrotron site	SSRF
Beamline	BL19U1
Temperature [K]	100
Detector technology	CCD
Collection date	2018-12-19
Detector	AGILENT ATLAS CCD
Wavelength(s)	0.97853
Spacegroup name	I 4
Unit cell lengths	142.221, 142.221, 107.422
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.720 - 1.700
R-factor	0.1593
Rwork	0.158
R-free	0.18210
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6j36
RMSD bond length	0.014
RMSD bond angle	1.285
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	19.960	1.790
High resolution limit [Å]	1.700	1.700
Rmeas	0.071	0.775
Number of reflections	115149	15087
<I/σ(I)>	18.6
Completeness [%]	98.3
Redundancy	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	277.15	0.14M Calcium chloride dihydrate, 0.07M Sodium acetate trihydrate pH 4.6, 14% v/v 2-Propanol, 30% v/v Glycerol