7E2O
X-ray Crystal structure of PPARgamma R288H mutant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-06-27 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 91.040, 60.920, 116.730 |
| Unit cell angles | 90.00, 102.24, 90.00 |
Refinement procedure
| Resolution | 114.080 - 3.200 |
| R-factor | 0.2377 |
| Rwork | 0.234 |
| R-free | 0.31330 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vv3 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.682 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | REFMAC |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 114.080 | 3.420 |
| High resolution limit [Å] | 3.200 | 3.200 |
| Number of reflections | 10209 | 1894 |
| <I/σ(I)> | 6.3 | |
| Completeness [%] | 97.5 | |
| Redundancy | 3.6 | |
| CC(1/2) | 0.968 | 0.927 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 298 | 0.1M Tris-HCl, 0.7M sodium citrate |
