7E1R
Crystal structure of Dehydrogenase/isomerase FabX from Helicobacter pylori in complex with holo-ACP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL19U1 |
| Synchrotron site | SSRF |
| Beamline | BL19U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2020-06-01 |
| Detector | MARMOSAIC 325 mm CCD |
| Wavelength(s) | 0.9785 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 51.793, 104.443, 99.576 |
| Unit cell angles | 90.00, 99.52, 90.00 |
Refinement procedure
| Resolution | 48.736 - 2.795 |
| R-factor | 0.2082 |
| Rwork | 0.206 |
| R-free | 0.24340 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4iql |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 99.576 | 2.900 |
| High resolution limit [Å] | 2.795 | 2.800 |
| Number of reflections | 21529 | 2310 |
| <I/σ(I)> | 15.5 | |
| Completeness [%] | 97.2 | |
| Redundancy | 6.6 | |
| CC(1/2) | 0.987 | 0.847 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7 | 292 | 0.1M HEPES pH 7.0, 20% w/v PEG 8000 |
