7E1J
Crystal structure of Pr55Gag-matrix domain in complex with IP6 in space group C121
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-05-02 |
| Detector | DECTRIS EIGER X 4M |
| Wavelength(s) | 1.10 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 176.182, 67.414, 97.499 |
| Unit cell angles | 90.00, 123.15, 90.00 |
Refinement procedure
| Resolution | 44.414 - 2.720 |
| R-factor | 0.2159 |
| Rwork | 0.213 |
| R-free | 0.27230 |
| Structure solution method | FOURIER SYNTHESIS |
| Data reduction software | XDS |
| Data scaling software | Aimless (0.5.31) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (DEV-3318) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 48.750 | 48.750 | 2.850 |
| High resolution limit [Å] | 2.720 | 9.020 | 2.720 |
| Rmerge | 0.099 | 0.037 | 0.802 |
| Rmeas | 0.107 | 0.040 | 0.867 |
| Rpim | 0.041 | 0.015 | 0.327 |
| Total number of observations | 177762 | 4854 | 23881 |
| Number of reflections | 25978 | 754 | 3438 |
| <I/σ(I)> | 14.3 | 44.6 | 2.4 |
| Completeness [%] | 99.9 | 99.1 | 99.9 |
| Redundancy | 6.8 | 6.4 | 6.9 |
| CC(1/2) | 0.998 | 0.999 | 0.881 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 277 | PEG 3350 and 100 mM MES |
