7E0M
Crystal structure of phospholipase D
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2020-11-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9791 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.780, 97.570, 117.030 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 23.880 - 1.790 |
| R-factor | 0.1703 |
| Rwork | 0.170 |
| R-free | 0.18480 |
| Structure solution method | SAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.469 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.18.1_3865) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.810 | 1.860 |
| High resolution limit [Å] | 1.790 | 1.790 |
| Rmerge | 0.134 | |
| Rpim | 0.042 | 0.393 |
| Number of reflections | 82957 | 5319 |
| <I/σ(I)> | 14.3 | 3 |
| Completeness [%] | 99.0 | 95.1 |
| Redundancy | 12.2 | 9.2 |
| CC(1/2) | 0.685 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 291 | 1.2 M Diammonium hydrogen phosphate, 0.1M imidazole/HCl pH7.8, 0.2M NaCl |
