7E06

Trans-3/4-proline-hydroxylase H11 with AKG

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2017-04-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 41 21 2
Unit cell lengths	106.763, 106.763, 144.010
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	85.760 - 1.970
R-factor	0.1708
Rwork	0.169
R-free	0.20400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6LNS
RMSD bond length	0.024
RMSD bond angle	2.132
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	85.760	2.000
High resolution limit [Å]	1.970	1.970
Rmerge	0.131	0.512
Rmeas	0.134	0.523
Rpim	0.027	0.106
Number of reflections	55651	2892
<I/σ(I)>	238.7
Completeness [%]	99.2	100
Redundancy	24.3	24
CC(1/2)		0.973

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	289.15	0.7M Sodium citrate tribasic dihydrate, 0.1M Bis-Tris propane (pH 7.0)