7E01
Trans-3/4-proline-hydroxylase H11 in the sixth reaction state
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRF BEAMLINE BL17U |
Synchrotron site | SSRF |
Beamline | BL17U |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-10-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | P 41 2 2 |
Unit cell lengths | 75.367, 75.367, 144.753 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 75.370 - 1.720 |
R-factor | 0.2089 |
Rwork | 0.207 |
R-free | 0.23630 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6LNS |
RMSD bond length | 0.028 |
RMSD bond angle | 2.458 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 75.370 | 1.750 |
High resolution limit [Å] | 1.720 | 1.720 |
Rmerge | 0.048 | 0.588 |
Number of reflections | 44839 | 2214 |
<I/σ(I)> | 184.5 | |
Completeness [%] | 99.8 | 99.6 |
Redundancy | 12.9 | 13.3 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 289.15 | 0.7 M Sodium citrate tribasic dihydrate, 0.1 M Bis-Tris propane (pH 7.0) |