7DZH

intermediate of FABP with a delay time of 100 ns

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	FREE ELECTRON LASER
Source details	SACLA BEAMLINE BL3
Synchrotron site	SACLA
Beamline	BL3
Temperature [K]	293
Detector technology	CCD
Collection date	2020-12-04
Detector	MPCCD
Wavelength(s)	1.24
Spacegroup name	P 21 21 21
Unit cell lengths	57.710, 66.710, 69.040
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.870 - 1.650
R-factor	0.1774
Rwork	0.176
R-free	0.20050
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1lkk
Data reduction software	DIALS
Data scaling software	DIALS
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	1.710
High resolution limit [Å]	1.650	1.650
Number of reflections	33748	3307
<I/σ(I)>	70.3	4.8
Completeness [%]	100.0
Redundancy	92
CC(1/2)	0.970	0.510

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7	277	0.1 M potassium thiocyanate, pH 7.0, 30% PEG 2000 monomethyl ether