7DZ9
MbnABC complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL18U1 |
| Synchrotron site | SSRF |
| Beamline | BL18U1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-07 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.915, 69.065, 82.573 |
| Unit cell angles | 90.00, 93.59, 90.00 |
Refinement procedure
| Resolution | 43.640 - 2.200 |
| Rwork | 0.173 |
| R-free | 0.21930 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.938 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX (1.17.1_3660) |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.640 | 2.279 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.118 | |
| Number of reflections | 46642 | 4648 |
| <I/σ(I)> | 18.78 | |
| Completeness [%] | 99.4 | |
| Redundancy | 13.7 | |
| CC(1/2) | 0.998 | 0.971 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 289.15 | 2% Tacsimate pH7.0, HEPES pH7.5, 20% PEG3350 |
