7DYC
Crystal structure of the chemically synthesized mk2h_deltaMILPYS peptide homodimer in complex with malate
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL26B2 |
Synchrotron site | SPring-8 |
Beamline | BL26B2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2020-12-10 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 52.278, 52.278, 146.731 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 33.225 - 2.300 |
R-factor | 0.2291 |
Rwork | 0.226 |
R-free | 0.26170 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 7dxz |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.440 |
High resolution limit [Å] | 2.300 | 2.300 |
Number of reflections | 10931 | 1638 |
<I/σ(I)> | 17.17 | |
Completeness [%] | 99.7 | |
Redundancy | 21.19 | |
CC(1/2) | 0.998 | 0.857 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 2100 mM DL Malic acid pH 7.0 |