7DYB

Thermotoga maritima ferritin mutant-FLAL-L

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	CCD
Collection date	2020-12-10
Detector	RAYONIX MX-225
Wavelength(s)	0.987
Spacegroup name	C 1 2 1
Unit cell lengths	175.343, 54.647, 59.910
Unit cell angles	90.00, 109.94, 90.00

Refinement procedure

Resolution	56.317 - 1.779
R-factor	0.1958
Rwork	0.194
R-free	0.23470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	56.320	1.844
High resolution limit [Å]	1.779	1.780
Number of reflections	48748	3604
<I/σ(I)>	18.3
Completeness [%]	94.6
Redundancy	7.6
CC(1/2)	0.999	0.965

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7.5	293	200 mM NaCl, 20% PEG 3000 and 100 mM HEPES/NaOH