7DY8

Thermotoga maritima ferritin mutant-FLAL

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NFPSS BEAMLINE BL18U
Synchrotron site	NFPSS
Beamline	BL18U
Temperature [K]	100
Detector technology	CCD
Collection date	2019-11-04
Detector	RAYONIX MX-225
Wavelength(s)	0.979
Spacegroup name	P 21 21 21
Unit cell lengths	39.418, 85.981, 107.090
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.054 - 2.103
R-factor	0.2167
Rwork	0.212
R-free	0.26460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vlg
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	29.100	2.179
High resolution limit [Å]	2.103	2.103
Number of reflections	21568	2073
<I/σ(I)>	22.845
Completeness [%]	98.4
Redundancy	7.3
CC(1/2)	0.982	0.969

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION	7	293	0.1M Ca(Ac)2, 20% PEG 1000, and 100 mM Imidazole/HCl