7DXW

Crystal structure of the mk2h_deltaMIL peptide homodimer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL26B2
Synchrotron site	SPring-8
Beamline	BL26B2
Temperature [K]	100
Detector technology	CCD
Collection date	2020-07-21
Detector	RAYONIX MX225-HS
Wavelength(s)	1
Spacegroup name	H 3 2
Unit cell lengths	69.596, 69.596, 65.781
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	34.798 - 1.509
R-factor	0.1831
Rwork	0.182
R-free	0.19600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	7dxr
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.600
High resolution limit [Å]	1.500	1.500
Number of reflections	9804	1557
<I/σ(I)>	23.33
Completeness [%]	99.9
Redundancy	21.62
CC(1/2)	0.998	0.945

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	100 mM Imidazole Hydrochloric acid pH 8.0, 200 mM Lithium sulfate, 10% PEG 3000